8GYA

Crystal structure of Alongshan virus methyltransferase bound to Sinefungin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 42 21 2
Unit cell lengths	120.184, 120.184, 88.421
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.491 - 2.005
R-factor	0.1994
Rwork	0.198
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8gy4
RMSD bond length	0.008
RMSD bond angle	1.127
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.124	0.891
Number of reflections	43931	4311
<I/σ(I)>	23.625
Completeness [%]	100.0
Redundancy	21.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.5M Lithium sulfate, 0.1M Tris pH 8.5, 25% w/v PEG 3350