8GY1

Crystal structure of Ag+ binding to Dendrorhynchus zhejiangensis ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL17B
Synchrotron site	NFPSS
Beamline	BL17B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-25
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.979
Spacegroup name	I 4 3 2
Unit cell lengths	148.212, 148.212, 148.212
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	104.800 - 1.900
R-factor	0.15273
Rwork	0.151
R-free	0.18965
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	1.875
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	BALBES
Refinement software	REFMAC (ccp4-7.0.024)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	106.780	2.000
High resolution limit [Å]	1.900	1.900
Number of reflections	21003	3175
<I/σ(I)>	7.2
Completeness [%]	99.7
Redundancy	11.3
CC(1/2)	0.990	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291.15	DzFer crystals were grown in 0.2 M ammonium sulfate, 0.1 M sodium acetate trihydrate pH 4.6, 30% w/v PEG monomethyl ether 2000, followed by soaking for 10 min with 20% glycerol and 100 mM AgNO3.