8GXO

The crystal structure of CsFAOMT1 in complex with SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-26
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.978690
Spacegroup name	P 32 2 1
Unit cell lengths	99.899, 99.899, 99.030
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.260 - 1.740
R-factor	0.1869
Rwork	0.186
R-free	0.22050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c3y
RMSD bond length	0.007
RMSD bond angle	1.016
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.740	1.740
Number of reflections	53776	847
<I/σ(I)>	23.8
Completeness [%]	99.0
Redundancy	20
CC(1/2)	1.000	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.05 M Cadmium Sulfate hydrate, 0.1 M HEPES pH 7.5, 1.0 M Sodium Acetate