8GXD

L-LEUCINE DEHYDROGENASE FROM EXIGUOBACTERIUM SIBIRICUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER D8 QUEST
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-02
Detector	Bruker PHOTON II
Wavelength(s)	1.54178
Spacegroup name	I 4 2 2
Unit cell lengths	152.990, 152.990, 132.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.620 - 3.020
R-factor	0.2156
Rwork	0.213
R-free	0.25520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6acf
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.620	3.200
High resolution limit [Å]	3.020	3.020
Number of reflections	15638	2418
<I/σ(I)>	9.5
Completeness [%]	99.5
Redundancy	5.2
CC(1/2)	0.986	0.699

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2M calcium chloride, 0.1M sodium acetate buffer (pH 4.6), 20% glycerol (w/v)