8GX2

The crystal structure of human CtsL in complex with 14c

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 2 21 21
Unit cell lengths	57.760, 76.270, 109.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.350 - 2.000
R-factor	0.1961
Rwork	0.194
R-free	0.23160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f06
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.350	44.350	2.200
High resolution limit [Å]	2.000	40.000	2.000
Rmerge	0.332	2.387	1.422
Rmeas	0.343	2.564	1.465
Total number of observations	566046
Number of reflections	33284	3	8131
<I/σ(I)>	7.41	0.26	2.73
Completeness [%]	100.0	42.9	100
Redundancy	17.007	6.667	17.343
CC(1/2)	0.995		0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	100mM sodium acetate (pH 4.1), 15% (w/v) PEG 2000, 8mg/ml protein