8GVI

The complex between H25-11 TCR and HLA-A24 bound to HIV-1 Nef138-8 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 32 2 1
Unit cell lengths	70.524, 70.524, 384.253
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.810 - 3.300
R-factor	0.2879
Rwork	0.286
R-free	0.31350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vxm
RMSD bond length	0.002
RMSD bond angle	0.545
Data reduction software	xia2
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.420
High resolution limit [Å]	3.300	3.300
Rmerge	0.161	2.033
Number of reflections	17740	17740
<I/σ(I)>	18.477	1.406
Completeness [%]	99.5
Redundancy	16.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Potassium chloride, 0.1 M tris (pH 8.0), 15% PEG2000 MME