8GVG
The complex between public TCR TD08 and HLA-A24 bound to HIV-1 Nef138-8 (2F) peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 71.035, 71.035, 383.869 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.940 - 3.370 |
R-factor | 0.3004 |
Rwork | 0.299 |
R-free | 0.31990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vxm |
RMSD bond length | 0.002 |
RMSD bond angle | 0.505 |
Data reduction software | xia2 |
Refinement software | REFMAC (5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.480 |
High resolution limit [Å] | 3.360 | 3.360 |
Rmerge | 0.127 | 0.968 |
Number of reflections | 16829 | 16829 |
<I/σ(I)> | 22.933 | 5.539 |
Completeness [%] | 98.9 | 98.4 |
Redundancy | 16.9 | 12.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Sodium sulfate, 0.1 M Bis-Tris Propane (pH 7.5), 20% PEG 3350 |