8GUM

Chitin-active AA10 LPMO (GbpA) from Vibrio campbellii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2021-04-23
Detector	RAYONIX MX-300
Wavelength(s)	0.99764
Spacegroup name	P 41 21 2
Unit cell lengths	113.612, 113.612, 215.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.100 - 2.350
R-factor	0.222
Rwork	0.220
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xwx
RMSD bond length	0.009
RMSD bond angle	0.995
Data reduction software	XDS
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.390
High resolution limit [Å]	2.350	2.350
Rmerge	0.092	1.595
Rmeas	0.096	1.680
Rpim	0.025	0.514
Number of reflections	59698	2937
<I/σ(I)>	28	0.8
Completeness [%]	99.9	99.7
Redundancy	14.3	10
CC(1/2)		0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292.15	1.0M Ammonium phosphate dibasic; 0.1M ADA buffer, pH 6.5