8GTX

Crystal Structure of human Spindlin1-HBx complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	47.788, 67.096, 93.695
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.850 - 1.800
R-factor	0.1647
Rwork	0.163
R-free	0.19670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mzg
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.101	0.048	0.577
Rmeas	0.110	0.052	0.627
Rpim	0.043	0.021	0.243
Total number of observations	185436
Number of reflections	28755	1589	1431
<I/σ(I)>	4.9
Completeness [%]	99.9	99.7	100
Redundancy	6.4	6	6.5
CC(1/2)		0.997	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	16%(w/v) PEG 8000, 0.04M Potassium phosphate dibasic, 20% (v/v) Glycerol