8GRV

Dictyostelium discoideum Lactate dehydrogenase (DicLDHA)with NAD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2020-11-23
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	P 64 2 2
Unit cell lengths	104.499, 104.499, 163.371
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.803 - 2.900
R-factor	0.1791
Rwork	0.175
R-free	0.26050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wn9
RMSD bond length	0.007
RMSD bond angle	0.954
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.500	2.980
High resolution limit [Å]	2.900	2.920
Number of reflections	21758	999
<I/σ(I)>	3
Completeness [%]	99.9
Redundancy	12.5
CC(1/2)	0.864	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		291	1.9M Ammonium sulfate, 90 mM Sodium acetate pH4.6