8GRJ

Crystal structure of gamma-alpha subunit complex from Burkholderia cepacia FAD glucose dehydrogenase in complex with gluconolactone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-13
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 65 2 2
Unit cell lengths	111.235, 111.235, 527.106
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.040 - 2.950
R-factor	0.2018
Rwork	0.198
R-free	0.27020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a2u
RMSD bond length	0.007
RMSD bond angle	1.705
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	3.000
High resolution limit [Å]	2.950	8.010	2.950
Rmerge	0.113	0.054	0.424
Total number of observations	653811
Number of reflections	42134	2284	2055
<I/σ(I)>	7.4
Completeness [%]	99.4	93	100
Redundancy	15.5	13.6	16.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	Tacsimate