8GR5

Cop4 from Antrodia cinnamomea in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2019-12-21
Detector	RAYONIX MX-300
Wavelength(s)	0.9998
Spacegroup name	C 1 2 1
Unit cell lengths	68.239, 59.768, 85.221
Unit cell angles	90.00, 110.90, 90.00

Refinement procedure

Resolution	27.980 - 2.100
R-factor	0.1947
Rwork	0.189
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ofl
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Rmeas	0.133
Number of reflections	18357	1827
<I/σ(I)>	15
Completeness [%]	99.0
Redundancy	6.6
CC(1/2)		0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	HEPES pH 7.5, 10% 2-Propanol, 20% PEG 4000