8GPX

YFV_E_YD73Fab_postfusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 3 2 1
Unit cell lengths	102.874, 102.874, 178.663
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.930 - 3.800
R-factor	0.2635
Rwork	0.261
R-free	0.30590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jhl
RMSD bond length	0.002
RMSD bond angle	0.540
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.940
High resolution limit [Å]	3.800	3.800
Rmerge	0.188	0.866
Number of reflections	11224	1088
<I/σ(I)>	8.082
Completeness [%]	99.4
Redundancy	3.6
CC(1/2)		0.627

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.2 M imidazole malate, pH 5.5, and 33% (v/v) PEG 600