8GOS
Crystal structure of fluorescent protein RasM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-21 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.394, 62.328, 107.819 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.998 - 1.010 |
| R-factor | 0.1391 |
| Rwork | 0.138 |
| R-free | 0.15840 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.315 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 1.030 |
| High resolution limit [Å] | 1.010 | 1.010 |
| Rmerge | 0.051 | 0.774 |
| Rpim | 0.022 | 0.349 |
| Number of reflections | 209327 | 10267 |
| <I/σ(I)> | 17.9 | 2.1 |
| Completeness [%] | 98.9 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 100 mM HEPES-NaOH buffer (pH 7.5) and 70% (v/v) MPD |
