8GOP

SARS-CoV-2 specific private TCR RLQ7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	52.178, 68.054, 63.878
Unit cell angles	90.00, 90.24, 90.00

Refinement procedure

Resolution	34.800 - 2.800
R-factor	0.2333
Rwork	0.231
R-free	0.28620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8gom
Data reduction software	XDS
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.800	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	10990	1107
<I/σ(I)>	9.39
Completeness [%]	94.5
Redundancy	9.39
CC(1/2)	0.842	0.647

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.02 M Sodium phosphate monobasic, 0.1M Bis-Tris propane pH 7.5, 20% PEG 3350