8GOK

Legionella OTU deubiquitinase LotA OTU1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97942
Spacegroup name	P 41 21 2
Unit cell lengths	86.217, 86.217, 91.701
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.480 - 1.520
R-factor	0.196
Rwork	0.195
R-free	0.21330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7f9x
RMSD bond length	0.006
RMSD bond angle	0.905
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.480	1.574
High resolution limit [Å]	1.520	1.520
Rmerge	0.017	0.457
Rmeas	0.025	0.646
Rpim	0.017	0.457
Number of reflections	53716	5296
<I/σ(I)>	18.91	1.56
Completeness [%]	100.0	99.94
Redundancy	2	2
CC(1/2)	1.000	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	290	35% (w/v) PEG 4000, 0.1M Tris-HCl [pH7.8], 0.8 M Lithium Chloride