8GOH
Homotetramic Antiparallel Coiled-Coil of 23-residues LacI C-terminal tetramerization helix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.257, 50.935, 40.849 |
| Unit cell angles | 90.00, 113.84, 90.00 |
Refinement procedure
| Resolution | 37.360 - 1.100 |
| R-factor | 0.2083 |
| Rwork | 0.207 |
| R-free | 0.22760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rzs |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.765 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.360 | 1.139 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.080 | 1.193 |
| Rmeas | 0.088 | 1.307 |
| Rpim | 0.034 | 0.524 |
| Number of reflections | 55084 | 5465 |
| <I/σ(I)> | 11.01 | 1.32 |
| Completeness [%] | 99.8 | 99.24 |
| Redundancy | 6.5 | 6 |
| CC(1/2) | 0.999 | 0.727 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 278 | 0.1M Na/K phosphate pH6.2, 0.2M Sodium chloride, 50% PEG200 |
