8GO5
Fungal immunomodulatory protein FIP-nha WT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-25 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 |
Unit cell lengths | 66.577, 74.467, 87.473 |
Unit cell angles | 99.33, 112.15, 108.59 |
Refinement procedure
Resolution | 25.686 - 2.809 |
R-factor | 0.2206 |
Rwork | 0.219 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7wdl |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.809 | 2.809 |
Rmerge | 0.082 | 0.320 |
Number of reflections | 33042 | 1675 |
<I/σ(I)> | 13.91 | 2.41 |
Completeness [%] | 96.8 | |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 22% PEG 1000, 0.1M Sodium citrate pH3.5, 0.2M Ammonium sulfate |