8GNN

Crystal structure of the human RAD9-RAD1-HUS1-RAD17 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.980
Spacegroup name	P 21 21 21
Unit cell lengths	53.188, 136.303, 154.046
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.806 - 2.119
R-factor	0.2188
Rwork	0.217
R-free	0.26250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j8y
RMSD bond length	0.003
RMSD bond angle	0.666
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.240
High resolution limit [Å]	2.119	2.119
Rmerge	0.067	0.916
Rmeas	0.073	0.887
Number of reflections	64368	6285
<I/σ(I)>	18.26	2.12
Completeness [%]	99.9	99.56
Redundancy	6.8
CC(1/2)	0.999	0.761

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Bis-Tris propane pH 7.5, sodium citrate, PEG 3350