8GN4

The crystal structure of ZBTB10 ZF1-2 R767Q in complex with telomeric DNA TTAGGG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	30.866, 32.665, 36.105
Unit cell angles	105.99, 90.46, 105.21

Refinement procedure

Resolution	30.188 - 1.900
R-factor	0.2125
Rwork	0.210
R-free	0.25910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8gn3
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.930
High resolution limit [Å]	1.900	5.150	1.900
Rmerge	0.039	0.023	0.300
Rmeas	0.046	0.027	0.362
Rpim	0.024	0.014	0.201
Total number of observations	31985
Number of reflections	9392	473	356
<I/σ(I)>	9
Completeness [%]	91.7	93.8	71.5
Redundancy	3.4	3.7	2.7
CC(1/2)		0.998	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Sodium Cacodylate, pH 6.5, 27 % v/v PEG 2000 MME