8GL7
AncAR1 - progesterone - Tif2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2023-02-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 69.714, 69.714, 144.439 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.300 - 2.400 |
| R-factor | 0.1999 |
| Rwork | 0.197 |
| R-free | 0.25270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.992 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 2.486 |
| High resolution limit [Å] | 2.372 | 2.400 |
| Rmeas | 0.423 | |
| Rpim | 0.088 | 0.411 |
| Number of reflections | 14581 | 4584 |
| <I/σ(I)> | 1.77 | |
| Completeness [%] | 97.0 | 91 |
| Redundancy | 22.5 | |
| CC(1/2) | 0.990 | 0.726 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 4000, 0.1M Tris pH 8.9, 4% glycerol |
