8GKJ

Crystal Structure of the Murine MUC16 Specific Antibody AR9.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-01
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	82.302, 96.820, 105.449
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.880 - 1.900
R-factor	0.184
Rwork	0.182
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.776
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.880	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.149
Rpim	0.041
Number of reflections	65609	6229
<I/σ(I)>	18.65
Completeness [%]	97.8
Redundancy	13.9
CC(1/2)	0.996	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	293	0.1M MES pH 5.7 6% tascimate pH 6.0 26% PEG 4000