8GJT

Crystal Structure of HIV-1 LM/HT Clade A/E CRF01 GP120 Core in Complex with TFH-I-116-D1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	64.670, 66.010, 87.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.770 - 3.500
R-factor	0.2563
Rwork	0.254
R-free	0.30660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.794
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.770	3.690
High resolution limit [Å]	3.500	3.500
Rpim	0.147	0.729
Number of reflections	4021	601
<I/σ(I)>	2.8	0.6
Completeness [%]	80.6
Redundancy	2.9	3
CC(1/2)	0.970	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	10% PEG 3350 5% PEG 400 0.1M HEPES pH 7.5