8GJH
Salmonella ArnA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.078 |
| Spacegroup name | P 1 |
| Unit cell lengths | 90.826, 129.439, 140.188 |
| Unit cell angles | 62.99, 71.66, 77.76 |
Refinement procedure
| Resolution | 29.830 - 3.600 |
| R-factor | 0.2194 |
| Rwork | 0.218 |
| R-free | 0.26690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z7e |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.730 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rpim | 0.098 | |
| Number of reflections | 59279 | 5929 |
| <I/σ(I)> | 7 | |
| Completeness [%] | 95.4 | 96 |
| Redundancy | 1.5 | 1.5 |
| CC(1/2) | 0.525 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 1.5 to 2.0 M ammonium sulfate and 0.1M MES pH 6.75 |
