8GIE

Crystal structure of a designed single-component Plasmodium falciparum AMA1-RON2L insertion fusion immunogen 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	40.630, 62.830, 60.470
Unit cell angles	90.00, 96.47, 90.00

Refinement procedure

Resolution	19.220 - 1.850
R-factor	0.1925
Rwork	0.191
R-free	0.21220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.626
Data reduction software	XDS (v Jan 10, 2022)
Data scaling software	XSCALE (v Jan 10, 2022)
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.220	1.916
High resolution limit [Å]	1.850	1.850
Rmerge	0.100	0.676
Rmeas	0.119	0.119
Rpim	0.064	0.426
Number of reflections	25370	2516
<I/σ(I)>	10.61	2.32
Completeness [%]	97.7	97.52
Redundancy	3.4	3.5
CC(1/2)	0.994	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291.15	0.5 M Lithium Chloride, 0.1 M Tris (pH 8.5), 34% (w/v) PEG 6000