8GEW
H-FABP crystal soaked in a bromo palmitic acid solution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-23 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.729320 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.736, 54.966, 70.747 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.470 - 0.970 |
| Rwork | 0.135 |
| R-free | 0.15300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.894 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.410 | 0.990 |
| High resolution limit [Å] | 0.970 | 0.970 |
| Rmerge | 0.051 | 0.847 |
| Number of reflections | 77518 | |
| <I/σ(I)> | 16.2 | 1.7 |
| Completeness [%] | 99.5 | 98.4 |
| Redundancy | 6.4 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20 mM Tris pH 7.5, 20-25% polyethylene glycol (PEG) 4000 |
