8GE0

Crystal structure of JADE1 PZP domain in complex with Histone H3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-08-24
Detector	RDI CMOS_8M
Wavelength(s)	1.27819
Spacegroup name	P 41 21 2
Unit cell lengths	78.160, 78.160, 222.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.510 - 2.400
R-factor	0.2086
Rwork	0.207
R-free	0.24120
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.647
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.510	2.490
High resolution limit [Å]	2.400	2.400
Rpim	0.028	0.388
Number of reflections	28040	2849
<I/σ(I)>	28.2
Completeness [%]	99.9
Redundancy	39.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2 M Sodium chloride, 0.1 M HEPES pH 7.0, 20 % w/v PEG 6000