8GCI

Crystal structure of C. elegans LIN-42 PAS-B domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	I 2 2 2
Unit cell lengths	50.733, 105.907, 152.966
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.940 - 2.420
R-factor	0.2657
Rwork	0.263
R-free	0.29290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gec
RMSD bond length	0.009
RMSD bond angle	1.083
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.940	2.510
High resolution limit [Å]	2.420	2.420
Rmerge	0.140	0.658
Number of reflections	16123	1634
<I/σ(I)>	10.2	3.3
Completeness [%]	99.5
Redundancy	7.1
CC(1/2)	0.994	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.15M Lithium sulfate, 1.5M sodium potassium tartrate