8GBL
Structure of Apo Human SIRT5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-04-17 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.624, 112.528, 56.083 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 41.620 - 2.240 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.620 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.081 | 0.756 |
| Number of reflections | 23939 | 1890 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 96.7 | |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES 6.5, 25% PEG 4K |
