8GBE
Structure of a viral gasdermin protein A47 from Eptesipox virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-12-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97911 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 64.465, 74.140, 95.599 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.650 - 1.460 |
| R-factor | 0.1915 |
| Rwork | 0.191 |
| R-free | 0.21160 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.096 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.650 | 1.480 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rpim | 0.019 | 0.718 |
| Number of reflections | 40114 | 1969 |
| <I/σ(I)> | 18.3 | 1.6 |
| Completeness [%] | 100.0 | 99.6 |
| Redundancy | 13.5 | 13.2 |
| CC(1/2) | 0.999 | 0.505 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | 100 mM ADA and 40% PEG-200 |
