8G9V
Crystal structures of 17-beta-hydroxysteroid dehydrogenase 13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.150, 161.560, 100.770 |
| Unit cell angles | 90.00, 95.05, 90.00 |
Refinement procedure
| Resolution | 37.910 - 2.645 |
| R-factor | 0.2136 |
| Rwork | 0.212 |
| R-free | 0.24430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.950 | 2.897 |
| High resolution limit [Å] | 2.645 | 2.645 |
| Rpim | 0.083 | 0.589 |
| Number of reflections | 67940 | 3398 |
| <I/σ(I)> | 7.9 | 1.5 |
| Completeness [%] | 93.4 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.990 | 0.462 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% PEG3350, 0.1 M Bis-Tris pH 5.5, 0.2M Li2SO4 |
