8G9P
Tricomplex of RMC-4998, KRAS G12C, and CypA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.03317 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.030, 101.760, 66.360 |
| Unit cell angles | 90.00, 91.04, 90.00 |
Refinement procedure
| Resolution | 43.430 - 1.500 |
| R-factor | 0.1579 |
| Rwork | 0.156 |
| R-free | 0.18750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.215 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.430 | 1.554 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.059 | 0.328 |
| Rmeas | 0.068 | 0.377 |
| Rpim | 0.033 | 0.183 |
| Number of reflections | 94582 | 8308 |
| <I/σ(I)> | 12.55 | 3.44 |
| Completeness [%] | 93.0 | 82.35 |
| Redundancy | 4.1 | 4.1 |
| CC(1/2) | 0.998 | 0.918 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 22% PEG 3350, 100 mM NaCl |
