8G93
Crystal structures of 17-beta-hydroxysteroid dehydrogenase 13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-04 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.730, 186.320, 65.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.090 - 1.910 |
| R-factor | 0.201 |
| Rwork | 0.200 |
| R-free | 0.22400 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | BUSTER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.160 | 2.210 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rpim | 0.065 | 0.404 |
| Number of reflections | 36866 | 1835 |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 93.6 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.997 | 0.564 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG3350, 0.2 M ammonium chloride |
