8G8V
GTP Cyclohydrolase-IB with sodium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-10-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 116.499, 116.499, 70.806 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.080 - 2.030 |
R-factor | 0.2012 |
Rwork | 0.199 |
R-free | 0.22770 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.669 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.20.1_4487) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.080 | 2.103 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmerge | 0.237 | 1.719 |
Rmeas | 0.243 | 1.771 |
Rpim | 0.055 | 0.424 |
Number of reflections | 36073 | 3546 |
<I/σ(I)> | 13.5 | 1.67 |
Completeness [%] | 99.9 | 99.77 |
Redundancy | 19.8 | 17 |
CC(1/2) | 0.998 | 0.826 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES Na, 1.6 M ammonium sulfate, 2% PEG 1000 |