8G8S

bipartite p52 NLS in complex with Importin alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	77.066, 88.583, 96.671
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.730 - 2.100
R-factor	0.1696
Rwork	0.169
R-free	0.18610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bw0
RMSD bond length	0.007
RMSD bond angle	0.803
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.730	2.300
High resolution limit [Å]	2.100	2.100
Number of reflections	39376	1917
<I/σ(I)>	10.6
Completeness [%]	100.0
Redundancy	9.4
CC(1/2)	0.997	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.01M DTT, 0.1M sodium HEPES pH7, 0.6M sodium citrate