8G8A
Crystal structure of DH1317.8 Fab in complex with HIV proximal MPER peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 164.976, 63.840, 111.971 |
Unit cell angles | 90.00, 101.06, 90.00 |
Refinement procedure
Resolution | 41.890 - 2.440 |
R-factor | 0.2238 |
Rwork | 0.222 |
R-free | 0.25590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nrx |
RMSD bond length | 0.005 |
RMSD bond angle | 0.848 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.530 |
High resolution limit [Å] | 2.440 | 2.440 |
Rmerge | 0.067 | 0.610 |
Number of reflections | 40649 | 3324 |
<I/σ(I)> | 16.8 | |
Completeness [%] | 94.8 | 77.4 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 20.1% Isopropanol, 10.05% PEG 8000, and 0.1M Imidazole/Hydrochloric acid pH 6.5 |