8G5X

Structure of the Class II Fructose-1,6-Bisphophatase from Francisella tularensis complexed with native metal cofactor Mn++ and substrate Fructose-1,6-Bisphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2022-12-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1
Unit cell lengths	63.760, 74.577, 77.191
Unit cell angles	67.89, 67.89, 77.01

Refinement procedure

Resolution	37.180 - 2.200
R-factor	0.1678
Rwork	0.165
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.700
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.059	0.386
Rmeas	0.527	0.527
Rpim	0.054	0.356
Number of reflections	59766	2923
<I/σ(I)>	20.92	2.21
Completeness [%]	97.3
Redundancy	2.2
CC(1/2)	0.997	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	Hampton Research PEG/Ion2 #30, 0.2 M Ammonium Tartrate dibasic, 20% PEG 3350