8G53

Crystal structure of a bacterial TPAT family transporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	52.945, 59.029, 62.543
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.340 - 1.030
Rwork	0.165
R-free	0.18290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.059
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.340	1.067
High resolution limit [Å]	1.030	1.030
Number of reflections	91316	5924
<I/σ(I)>	26.55
Completeness [%]	93.7
Redundancy	8.3
CC(1/2)	0.999	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Lithium Sulfate 0.1 M Tris: HCl, pH 8.5 1.26 M Ammonium Sulfate