8G4Y

Structure of ZNRF3 ECD bound to peptide MK1-3.6.10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 2.0.1
Synchrotron site	ALS
Beamline	2.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.036
Spacegroup name	C 1 2 1
Unit cell lengths	135.551, 35.226, 48.856
Unit cell angles	90.00, 109.11, 90.00

Refinement procedure

Resolution	32.020 - 1.410
R-factor	0.2145
Rwork	0.213
R-free	0.23610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.916
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.160	1.640
High resolution limit [Å]	1.410	1.550
Rmerge	0.056	0.748
Rmeas	0.062	0.834
Rpim	0.028	0.366
Total number of observations		22697
Number of reflections	35592	4445
<I/σ(I)>	14.4	2.5
Completeness [%]	97.3
Redundancy	5	5.1
CC(1/2)	0.998	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	277	0.1 M sodium citrate pH 4.5, 20% PEG 4000 plus silver bullet A5 (Hampton Research)