8G2M
The tumor activated anti-CTLA-4 monoclonal antibody XTX101 demonstrates tumor-growth inhibition and tumor-selective pharmacodynamics in mouse models of cancer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-03 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.180, 55.230, 73.860 |
| Unit cell angles | 90.00, 118.70, 90.00 |
Refinement procedure
| Resolution | 28.980 - 1.800 |
| R-factor | 0.18155 |
| Rwork | 0.180 |
| R-free | 0.21395 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.282 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.980 | 1.864 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.065 | 0.569 |
| Rmeas | 0.069 | |
| Rpim | 0.023 | 0.314 |
| Number of reflections | 42381 | 3390 |
| <I/σ(I)> | 21.67 | 2.05 |
| Completeness [%] | 97.0 | |
| Redundancy | 8.3 | |
| CC(1/2) | 0.999 | 0.848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Ammonium Citrate Dibasic pH 5.0, 20% PEG 3350 |
