8G2B

Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with iboxamycin, mRNA, deacylated A- and E-site tRNAphe, and aminoacylated P-site fMet-tRNAmet at 2.55A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-13
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	209.640, 449.560, 621.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	123.210 - 2.550
R-factor	0.2202
Rwork	0.218
R-free	0.26170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.832
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	123.210	123.210	2.620
High resolution limit [Å]	2.550	11.400	2.550
Rmerge	0.157	0.030	1.746
Rmeas	0.174	0.033	1.937
Total number of observations	9628355
Number of reflections	1845878	21273	136431
<I/σ(I)>	8.8	37.2	0.92
Completeness [%]	98.2	95.9	98.5
Redundancy	5.216	5.399	5.394
CC(1/2)	0.994	0.999	0.166

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME