8G1N
Structure of Campylobacter concisus PglC I57M/Q175M Variant with modeled C-terminus
Replaces: 5W7LExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.5498 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 70.802, 70.802, 188.442 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 62.810 - 2.740 |
R-factor | 0.2604 |
Rwork | 0.259 |
R-free | 0.29690 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.475 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELXD |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.814 | 2.870 |
High resolution limit [Å] | 2.740 | 2.740 |
Rmerge | 0.097 | 1.033 |
Rpim | 0.032 | 0.434 |
Number of reflections | 27735 | 1941 |
<I/σ(I)> | 21.4 | 2.5 |
Completeness [%] | 100.0 | |
Redundancy | 18.1 | 11.8 |
CC(1/2) | 1.000 | 0.714 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290.15 | Bis-Tris 0.1 M, magnesium chloride hexahydrate 0.4 M, PEG 3350 23% |