8G1B

Crystal structure of polyreactive 2G02 human Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	70.160, 59.440, 99.280
Unit cell angles	90.00, 98.83, 90.00

Refinement procedure

Resolution	69.330 - 1.670
R-factor	0.1948
Rwork	0.194
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.842
Data reduction software	iMOSFLM
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.330	1.730
High resolution limit [Å]	1.670	1.670
Number of reflections	92961	9082
<I/σ(I)>	7.22
Completeness [%]	96.4
Redundancy	1.9
CC(1/2)	0.943	0.171

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	20% PEG 3350, 0.2M Magnesium formate