8G19

Crystal structure of monoreactive 4C05 human Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	79.839, 70.609, 87.680
Unit cell angles	90.00, 108.95, 90.00

Refinement procedure

Resolution	39.540 - 1.770
R-factor	0.1886
Rwork	0.187
R-free	0.21940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.017
Data reduction software	iMOSFLM
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.540	1.833
High resolution limit [Å]	1.770	1.770
Number of reflections	44160	4398
<I/σ(I)>	11.61
Completeness [%]	98.0
Redundancy	1.9
CC(1/2)	0.998	0.924

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	30% PEG 5000 MME, 0.2M Ammonium sulfate, 0.1M MES pH 6.5