8G0P
Crystal structure of the human Ndc80:Nuf2 loop region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97910 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 49.903, 72.259, 80.065 |
| Unit cell angles | 90.00, 98.56, 90.00 |
Refinement procedure
| Resolution | 40.750 - 2.000 |
| R-factor | 0.262 |
| Rwork | 0.260 |
| R-free | 0.29680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.456 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.750 | 1.870 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.122 | 4.030 |
| Rmeas | 0.132 | 4.440 |
| Rpim | 0.050 | 1.820 |
| Number of reflections | 36742 | 1005 |
| <I/σ(I)> | 9.5 | 2.4 |
| Completeness [%] | 98.7 | 84.5 |
| Redundancy | 6.8 | 5.7 |
| CC(1/2) | 1.000 | 0.330 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% (w/v) polyethylene glycol 4000, 0.1 M TRIS pH 8.6, and 0.2 M magnesium chloride |
