8FZZ
Phocaeicola vulgatus type VI secretion system Ntox15 effector and immunity Tde2/Tdi2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-03-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.311, 84.769, 175.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.040 - 2.680 |
| R-factor | 0.1905 |
| Rwork | 0.185 |
| R-free | 0.25080 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.933 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (v1.20.1) |
| Refinement software | PHENIX (v1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.100 | 2.720 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.160 | 1.200 |
| Number of reflections | 42063 | 601 |
| <I/σ(I)> | 19.6 | 2 |
| Completeness [%] | 98.3 | 85.2 |
| Redundancy | 13.5 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 5% PEG 3350 (w/v), 200 mM NaCl, and 100 mM sodium citrate pH 5.0 |
