8FZP

Crystal structure of polyreactive 43G10 mouse Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	62.240, 108.470, 115.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.150 - 1.630
R-factor	0.2253
Rwork	0.224
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.874
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.150	1.692
High resolution limit [Å]	1.630	1.634
Number of reflections	97569	9583
<I/σ(I)>	18.9
Completeness [%]	99.3
Redundancy	23
CC(1/2)	0.741	0.094

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	16% PEG 4000, 0.2M Ammonium sulfate, 0.1M HEPES pH 7.5, 10% Isopropanol