8FXO
Bromodomain of CBP liganded with iCBP8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2021-10-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.540, 34.630, 40.440 |
| Unit cell angles | 90.00, 93.25, 90.00 |
Refinement procedure
| Resolution | 32.340 - 1.740 |
| R-factor | 0.1786 |
| Rwork | 0.176 |
| R-free | 0.20490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.042 |
| Data reduction software | XDS (Feb 5, 2021) |
| Data scaling software | XSCALE (Feb 5, 2021) |
| Phasing software | PHENIX (1.20.1_4487) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.340 | 1.790 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmeas | 0.034 | 0.130 |
| Number of reflections | 12730 | 842 |
| <I/σ(I)> | 26.8 | 7.6 |
| Completeness [%] | 97.4 | 88.9 |
| Redundancy | 4.7 | 3.7 |
| CC(1/2) | 0.999 | 0.992 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 0.2 M Sodium acetate trihydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4000 |
