8FXN

Bromodomain of CBP liganded with iCBP7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	93
Detector technology	PIXEL
Collection date	2021-10-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0032
Spacegroup name	P 32
Unit cell lengths	65.470, 65.470, 24.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.730 - 2.000
R-factor	0.2004
Rwork	0.197
R-free	0.24250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.223
Data reduction software	XDS (Feb 5, 2021)
Data scaling software	XSCALE (Feb 5, 2021)
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.730	2.050
High resolution limit [Å]	2.000	2.000
Rmeas	0.077	0.830
Number of reflections	7885	593
<I/σ(I)>	17.9
Completeness [%]	99.9	100
Redundancy	7	7.3
CC(1/2)	0.999	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.03M Sodium nitrate, 0.03 Sodium phosphate dibasic, 0.03M Ammonium sulfate, 0.1 M Imidazole, 0.1 M MES pH 6.5, 20% v/v PEG 500 MME; 10% w/v PEG 20000