8FWY

Crystal structure of the Trypanosoma cruzi hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGXPRT), isoform D, bound to the dead-end complex xanthine and pyrophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	120
Detector technology	PIXEL
Collection date	2018-04-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97937
Spacegroup name	P 21 21 21
Unit cell lengths	60.229, 81.015, 90.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.340 - 1.540
R-factor	0.1895
Rwork	0.189
R-free	0.19830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tc2
RMSD bond length	0.017
RMSD bond angle	1.755
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.340	1.570
High resolution limit [Å]	1.540	1.540
Rpim	0.040	0.494
Number of reflections	60863	1536
<I/σ(I)>	10.7	3.3
Completeness [%]	91.8
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.2 M lithium sufate, 24% PEG3350, 0.1 M HEPES, pH 8.0